Hi Kim, great question. Yes, you can make the Strawberry Shortcake Roll one day in advance, filling included. The cream cheese in the filling acts as a stabilizer, so it holds its shape and doesn’t get runny or watery overnight. Just dry the strawberries well and wrap the roll tightly before refrigerating. It’s fine to serve cold, but letting it sit out for 15 minutes helps the cake soften slightly and makes slicing easier.